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Journal of Biomedicine and Biotechnology
Volume 2012, Article ID 737134, 6 pages
http://dx.doi.org/10.1155/2012/737134
Research Article

AngiotensinII Preconditioning Promotes Angiogenesis In Vitro via ERKs Phosphorylation

1Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University, 180 Feng Lin Road, Shanghai 200032, China
2Institutes of Biological Science, Fudan University, 138 Yixueyuan Road, Shanghai 200032, China
3Institute of Shanghai Medical Science and Technology Information, 602 West Jianguo Road, Shanghai 200032, China

Received 26 September 2011; Revised 3 November 2011; Accepted 21 November 2011

Academic Editor: Yujian James Kang

Copyright © 2012 Aili Guan et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

AngiotensinII (AngII) is involved in not only the formation of cardiac hypertrophy but also the development of cardiac remodeling both of which are associated with myocardial angiogenesis. This study was therefore performed to clarify the effects of AngII on the formation of vasculatures by cultured cardiac microvascular endothelial cells (CMVECs) after a long-period stimulation with or without the AngII preconditioning. Incubation with AngII for 18 hrs significantly impaired the formation of capillary-like tubes comparing to that without AngII. CMVECs with AngII pretreatment for 5 and 10 min formed more capillary-like tubes than those without AngII pretreatment, suggesting that preconditioning with AngII at a lower dose for a short period could prevent the further damage of CMVECs by a higher concentration of AngII. Moreover, AngII ( 1 0 7  M) stimulation for 5 and 10 min significantly induced the increase in extracellular signal-regulated protein kinases (ERKs) phosphorylation, and an ERKs inhibitor, PD98059, abrogated the increase in the formation of capillary-like tubes induced by the AngII-pretreatment. In conclusion, preconditioning with a lower concentration of AngII for a short period prevents the subsequent impairment of CMVECs by a higher dose of AngII, at least in part, through the increase in ERKs phosphorylation.