AngiotensinII Preconditioning Promotes Angiogenesis In Vitro via ERKs Phosphorylation
Figure 3
The effects of PD98059 on ERKs phosphorylation in cultured CMVECs. CMVECs were incubated with (+) or without (−) PD98059 (), an ERKs inhibitor, for 30 min and then treated with (+) or without (−) AngII (10−7 M) for 10 min. Thereafter, the cells were collected and lysed for western blotting. (a) Representative immunoblots showing the expression of phosphor ERKs. GAPDH was used as a loading control. (b) Quantitative analysis for the expression of phosphor ERKs. Data represent mean ± SD obtained from 4 independent experiments (). * versus CMVECs with neither PD98059 nor AngII treatment; versus CMVECs without PD98059 but with AngII treatment.
