Figure 1: Recognition of PLG binding to Leptospira by L-IFA. Live virulent L. interrogans serovar Copenhageni isolates were treated with PLG, and the recognition was assessed through polyclonal anti-PLG antibodies under a confocal immunofluorescence microscope (PLG 1 to 3). PLG-treated leptospires stained for the proteins LipL32 (outer surface protein marker) or GroEL (a protoplasmic-cylinder marker) and untreated leptospires (PBS), are shown as controls (a) DNA propidium iodide-stained, (b) FITC-stained and (c) A+B composite images.