Figure 10: Human IgG and C3b binding to L. interrogans. Bacteria were coated onto microtiter plates (25 × 106 leptospires/well) and IgG (a) or C3b (b)were bound by incubating bacteria with 100% NHS. Bacteria were treated with PBS, 40 μg/mL PLG (PLG), 5 μg/mL uPA (uPA), or PLG + uPA (Pla). Presence of human IgG or C3b deposited on leptospires was determined by ELISA. Bars represent the mean absorbance values at 492 nm ± the standard deviation of three replicates for each experimental group and are representative of two independent experiments.