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Journal of Biomedicine and Biotechnology
Volume 2012, Article ID 760679, 8 pages
Research Article

Aberrant Expression of N-Methylpurine-DNA Glycosylase Influences Patient Survival in Malignant Gliomas

1Department of Neurosurgery, The 309th Hospital of PLA, Beijing 100091, China
2Department of Emergency, Tangdu Hospital, The Fourth Military Medical University, Xi'an 710038, China
3Administrative Department, Tangdu Hospital, The Fourth Military Medical University, Xi'an 710038, China
4Department of Neurosurgery, 254th Hospital of PLA, Tianjin 300142, China
5Department of Neurosurgery, Tangdu Hospital, The Fourth Military Medical University, Xi'an 710038, China

Received 27 November 2011; Revised 1 January 2012; Accepted 18 January 2012

Academic Editor: Paul W. Doetsch

Copyright © 2012 Ce Liu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Aim. To examine the expression of N-methylpurine-DNA glycosylase (MPG) gene and protein in glioma samples with different WHO grades and its association with patients’ survival. Methods. Immunohistochemistry assay, quantitative real-time PCR and Western blot analysis were carried out to investigate the expression of MPG gene and protein in 128 glioma and 10 non-neoplastic brain tissues. Results. MPG gene expression level in glioma tissues was significantly higher than that in non-neoplastic brain tissues ( 𝑃 < 0 . 0 0 1 ). Immunohistochemistry also showed that MPG protein was over-expressed in glioma tissues, which was consistent with the resutls of Western blot analysis. Additionally, the expression levels of MPG gene and protein both increase from grade I to grade IV glioma according to the results of real-time PCR, immunohistochemistry and western blot analysis. Moreover, the survival rate of MPG-positive patients was significantly lower than that of MPG-negative patients ( 𝑃 < 0 . 0 0 1 ). We further confirmed that the over-expression of MPG was a significant and independent prognostic indicator in glioma by multivariate analysis ( 𝑃 < 0 . 0 0 1 ). Conclusions. Our data showed the over-expression of MPG gene and protein in human gliomas, and also suggested for the first time that MPG be an unfavorable independent prognostic indicator for glioma patients.