Generation of Biotechnology-Derived Flavobacterium columnare Ghosts by PhiX174 Gene E-Mediated Inactivation and the Potential as Vaccine Candidates against Infection in Grass Carp

<table>Physical map of the lysis plasmid pBV E cat. Gene E, under transcriptional control of the temperature-sensitive repressor; rrBT1T2: ribosome rrnB gene providing translation stop signal terminator sequence; pro: the regulating sequence including promoter; cat: chloramphenicol acetyl transferase gene; ori: replication origin; cIts857: restraining gene of lambda bacteriophage adapted to heat induced expression.</table>

BioMed Research International

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Figure 1

Figure 1: Generation of Biotechnology-Derived Flavobacterium columnare Ghosts by PhiX174 Gene E-Mediated Inactivation and the Potential as Vaccine Candidates against Infection in Grass Carp

Figure 1 | Generation of Biotechnology-Derived Flavobacterium columnare Ghosts by PhiX174 Gene E-Mediated Inactivation and the Potential as Vaccine Candidates against Infection in Grass Carp 