Generation of Biotechnology-Derived <i>Flavobacterium columnare</i> Ghosts by PhiX174 Gene <i>E</i>-Mediated Inactivation and the Potential as Vaccine Candidates against Infection in Grass Carp

<table>Growth and lysis kinetics of <i>F. columnare</i> G4cpN22 harboring plasmid pBV E cat by temperature induction of gene <svg height="10.325" id="M8" style="vertical-align:-0.0pt" version="1.1" viewbox="0 0 12.225 10.325" width="12.225" xmlns="http://www.w3.org/2000/svg">
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<tspan style="font-size: 12.50px; " x="0" y="0">𝐸</tspan>
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</svg> expression. At time zero, the growth temperature of three bottles of the cultures was shifted from 26°C to 42°C (filled symbols), whereas the others served as noninduced control at 26°C (open symbols). Growth and lysis were monitored by the measurement of the OD<sub>600</sub> (open circles and filled circles) and the determination of the number of CFU (open squares and filled squares and discontinuous lines).</table>

BioMed Research International

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Figure 2

Figure 2: Generation of Biotechnology-Derived <i>Flavobacterium columnare</i> Ghosts by PhiX174 Gene <i>E</i>-Mediated Inactivation and the Potential as Vaccine Candidates against Infection in Grass Carp 

Figure 2 | Generation of Biotechnology-Derived Flavobacterium columnare Ghosts by PhiX174 Gene E-Mediated Inactivation and the Potential as Vaccine Candidates against Infection in Grass Carp 