Camel Milk Modulates the Expression of Aryl Hydrocarbon Receptor-Regulated Genes, Cyp1a1, Nqo1, and Gsta1, in Murine hepatoma Hepa 1c1c7 Cells

Figure 5

Effect of camel milk on XRE-dependent luciferase activity. Hepa 1c1c7 cells were transiently cotransfected with XRE-driven luciferase reporter plasmid pGudLuc 1.1. and renilla luciferase control plasmid pRL-CMV. Cells were treated with sterile water or camel milk (fat free, 100 𝜇L/mL) 30 min before the addition of TCDD (1 nM) for an additional 24 h. Cells were lysed and luciferase activity was reported as relative light unit (RLU) of firefly luciferase to renilla luciferase (Fluc/Rluc) (mean ± S.E.M., 𝑛=4). ⁺𝑃<0.05 compared control (sterile water-treated cells), ^∗𝑃<0.05 compared with TCDD-treated cells.