A Limited Role of p53 on the Ability of a Hexane Fraction of American Ginseng to Suppress Mouse Colitis
Figure 4
The HAG induces a G1 checkpoint in colon cancer cells in both p53+/+ and p53−/− colon cancer cells. (a) HCT-116 WT and p53−/− cells were exposed to HAG in RPMI-1640 + 1% NBCS for 24 hr at indicated doses and harvested for cell cycle assay with DAPI stain. Representative percentage of cells in G1, S, and G2/M-phases are indicated for each treatment. A minimum of 20,000 events was counted by flow cytometry from each treatment. (b) Confirmation of p53 protein status of the HCT-116 cells. Cell lysates were analyzed by western blot analysis. C+ indicates the positive control for p53, which is an archived HCT-116 cell lysate with wild-type p53. C− indicates the negative control for p53, which is an archived HCT-116 p53−/− cell lysate with p53 knockout.
