Figure 5: Gene expression analysis upon LMP silencing. The histogram represents the relative expression levels of LMP (24 and 48 hours following siRNA transfection, (a)), KLF2 and KLF4 (24 hours after LMP silencing, (b)). Values represent the mean of triplicate experiments for each test. SHAM: cells treated with transfection reagent alone; Neg Ctrl: cells transfected with AllStars Negative Control SiRNA; SiRNA-LMP: cells treated with SiRNA-LMP construct; RQ: relative quantity, calculated according to 2ΔΔCt method; *: statistically significant ().