Lim Mineralization Protein 3 Induces the Osteogenic Differentiation of Human Amniotic Fluid Stromal Cells through Kruppel-Like Factor-4 Downregulation and Further Bone-Specific Gene Expression

<table>Gene expression analysis upon LMP silencing. The histogram represents the relative expression levels of LMP (24 and 48 hours following siRNA transfection, (a)), KLF2 and KLF4 (24 hours after LMP silencing, (b)). Values represent the mean of triplicate experiments for each test. SHAM: cells treated with transfection reagent alone; Neg Ctrl: cells transfected with AllStars Negative Control SiRNA; SiRNA-LMP: cells treated with SiRNA-LMP construct; RQ: relative quantity, calculated according to 2<sup><i>‒</i>ΔΔ<i>Ct</i></sup> method; *: statistically significant (<span class="inline" id="M19"></span>).</table>

BioMed Research International

fig5

Figure 5

Figure 5: Lim Mineralization Protein 3 Induces the Osteogenic Differentiation of Human Amniotic Fluid Stromal Cells through Kruppel-Like Factor-4 Downregulation and Further Bone-Specific Gene Expression 

Figure 5 | Lim Mineralization Protein 3 Induces the Osteogenic Differentiation of Human Amniotic Fluid Stromal Cells through Kruppel-Like Factor-4 Downregulation and Further Bone-Specific Gene Expression 