BioMed Research International / 2012 / Article / Tab 1 / Research Article
Lim Mineralization Protein 3 Induces the Osteogenic Differentiation of Human Amniotic Fluid Stromal Cells through Kruppel-Like Factor-4 Downregulation and Further Bone-Specific Gene Expression Table 1 Sequences of oligonucleotide primer used in qPCR.
Gene symbol Sense* Sequence B-ACT Fw 5′ -TCGTGCGTGACATTAAGGAG-3′ Rv 5′ -CCATCTCTTGCTCGAAGTCC-3′ BMP2 Fw 5′ -CGTGTCCCCGCGTGCTTCTT-3′ Rv 5′ -CGGCCACCATGGTCGACCTT-3′ KLF2 Fw 5′ -GGCTTGTGATGCCTTGTGA-3′ Rv 5′ -TGCCCACCTGTCTCTCTATGT-3′ KLF4 Fw 5′ -GACCACCTCGCCTTACACAT-3′ Rv 5′ -CCCCCAACTCACGGATATAA-3′ LMP Fw 5′ -AGCATCGATGGCGAGAAT-3′ Rv 5′ -CCGGATCTTGTTCTGAGCTT-3′ OC Fw 5′ -GACTGTGACGAGTTGGCTGA-3′ Rv 5′ -AGCAGAGCGACACCCTAGAC-3′ OP Fw 5′ -TGAAACGAGTCAGCTGGATG- 3′ Rv 5′ -GCTCTCATCATTGGCTTTCC-3′ OSX Fw 5′ -TAGACACCATGACCCCATCA-3′ Rv 5′ -CATGGCAACAGGGGATTAAC-3′ RunX2 Fw 5′ -GATGTGCCTAGGCGCATT-3' Rv 5′ -AAAAGGGCCCAGTTCTGA-3′ SMARCC2 Fw 5′ -CAGACATCTCTCCCCCTCAC-3′ Rv 5′ -CTGGAACCGTGATGTCCAC-3′
* : Fw: forward (left) primer; Rv: reverse (right) primer.
