Research Article

Administration of Bone Marrow Derived Mesenchymal Stem Cells into the Liver: Potential to Rescue Pseudoxanthoma Elasticum in a Mouse Model (Abcc6−/−)

Figure 1

Characterization of the mouse bone marrow derived stem cells. The bone marrow derived stem cells were isolated by magnetic cell sorting using a Lin cocktail of antibodies as well as stem cell antigen-1 (Sca-1) antibody and then cultured in the regular stem cell culture medium. (a) The cells were observed by phase contrast microscopy at passage 3 (left) or passage 5 (right). The cells at passage 5 became more fibroblastic appearing compared to passage 3. (b) Flow cytometry was utilized to examine the surface markers of cells at passage 3 (top panels) or passage 5 (bottom panels) for the purity of cell population.
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(a)
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(b)