Molecular Characterization of a Fully Human Chimeric T-Cell Antigen Receptor for Tumor-Associated Antigen EpCAM
Figure 3
The results of the analysis of CAR57-28ζ protein expression. CAR57-28ζ-grafted Jurkat cells after hygromycin selection were analyzed by flow cytometry (a). Lysates were prepared from wild-type and CAR-expressing Jurkat cells, and immunoreactive bands were detected by a Western blotting analysis using an anti-CD3ζ antibody (b).
