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Journal of Biomedicine and Biotechnology
Volume 2012, Article ID 873090, 11 pages
http://dx.doi.org/10.1155/2012/873090
Research Article

Biochemical and Molecular Analysis of Some Commercial Samples of Chilli Peppers from Mexico

1Departamento de Ingenieria Bioquimica, Escuela Nacional de Ciencias Biológicas-Instituto Politécnico Nacional, Carpio y Plan de Ayala s/n Col, Casco de Santo Tomás, 11340 Mexico City, DF, Mexico
2Centro de Investigacion en Biotecnologia Aplicada, 90700 Exhacienda San Juan Molino Carretera Estatal Tecuexcomac-Tepetitla Km 1.5, TLAX, Mexico
3Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas-Instituto Politécnico Nacional, Carpio y Plan de Ayala s/n Col, Casco de Santo Tomás, 11340 Mexico City, DF, Mexico
4Departamento de Biotecnología Agrícola, CIIDIR-IPN, Juan de Dios Batíz Paredes 250, Guasave, 81101 Sinaloa, Mexico

Received 20 December 2011; Revised 1 March 2012; Accepted 6 March 2012

Academic Editor: P. B. Kirti

Copyright © 2012 Ivonne Guadalupe Troconis-Torres et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The genus Capsicum provides antioxidant compounds, such as phenolics and carotenoids, into the diet. In Mexico, there is a wide diversity of species and varieties of chilli peppers, a fruit which has local cultural and gastronomic importance. In the present study, the relationship of the carotenoid and phenolic profiles with the RAPD fingerprint of three different commercial cultivars of chilli peppers of seven regions of Mexico was investigated. Through RAPD, the species of chilli were differentiated by means of different primers (OPE-18, MFG-17, MFG-18, C51, and C52). The genetic distance found with OPE 18 was in the order of 2.6. The observed differences were maintained when the chromatographic profile of carotenoids, and the molecular markers were analyzed, which suggest a close relationship between carotenoids and the genetic profile. While the chromatographic profile of phenols and the molecular markers were unable to differentiate between genotypes of chilli peppers. In addition, by using infrared spectroscopy and statistical PCA, differences explained by geographic origin were found. Thus, this method could be an alternative for identification of chilli species with respect to their geographic origin.