The Immunogenicity of the Tumor-Associated Antigen α-Fetoprotein Is Enhanced by a Fusion with a Transmembrane Domain
Figure 2
AFP-specific CD8+ T-cell immune response after vaccination of C3H/HeN mice with MVA-s mAFP, MVA-I mAFP, or MVA-T mAFP. (a) Schematic of the experimental design; (b) IFNγ ELISpot assay, CD8+ T cells from mice vaccinated with MVA-null (♦: negative control) or MVA expressing the secreted (⚫), the intracellular (▲), or the transmembrane (■) form of murine AFP protein were stimulated in vitro by a specific AFP peptide. Statistical analyses were performed by the Kruskal-Wallis test.
