Persistent Dystrophin Protein Restoration 90 Days after a Course of Intraperitoneally Administered Naked 2′OMePS AON and ZM2 NP-AON Complexes in mdx Mice
Figure 2
Immunofluorescence analysis. (a) Restored dystrophin is detectable and correctly expressed at the membrane of muscle fibres (quadriceps and diaphragm) from ZM2–AON-treated mdx mice. Traces are also visible in skeletal muscles from naked AON-treated mice, although the distribution at the sarcolemma is not continuous and very faint. Cardiac muscles were found to be negative for restored dystrophin. (Scale bar = 100 μm in quadriceps, 50 μm in diaphragms, 25 μm in heart). (b) Here are represented some enlarged details of immunofluorescence images that highlight the different expression patterns of rescued dystrophin in the quadriceps and diaphragm of ZM2–AON- and naked AON-treated mdx mice. These images show both a brighter dystrophin labelling and a more continuous distribution along the fibre membranes in muscles from ZM2–AON-treated mice (NT, untreated; WT, wild type).
