Microbial Communities and Their Performances in Anaerobic Hybrid Sludge Bed-Fixed Film Reactor for Treatment of Palm Oil Mill Effluent under Various Organic Pollutant Concentrations
Table 3
The partial 16S rRNA gene sequences of ARC domain and organism with the best-matching sequences determined by BLAST searches.
Affiliation
DGGE band
Similarity (%)
Accession no.
Seed
C1
C2
C3
Acetoclastic methanogens
Uncultured Methanococcoides sp.
G5
I8
J9
K11
L12
M8
89
AY454739.1
Uncultured Methanosaeta sp.
G3
H6
I6
J6
K8
L9
M6
85
AY454766.1
Methanocaldococcus vulcanius M7
I2
K2
L2
M2
90
CP001787.1
Hydrogenotrophic methanogens
Methanobacterium sp.
G1
H4
I4
J4
K6
L7
M4
93
GU936489.1
Methanobacterium sp.
G2
H5
I5
J5
K7
L8
M5
95
GU569395.1
Methanomicrobiaceae
G4
H7
I7
J7
K9
L10
M7
87
GU129124.1
Uncultured Methanomicrobiales
H1
I1
J1
K1
L1
M1
95
AY780566.1
Methanospirillum hungatei
H2
99
AB517987.1
Methanobacterium sp.
H3
I3
J3
K4
L4
M3
97
GU936489.1
Methanobacteriaceae
K5
89
GU129060.1
Methanoculleus sp.
J8
K10
L11
96
AB436897.1
Methanobacterium palustre
L5
91
EU293795.1
Methanobacterium sp.
L6
95
GU569395.1
Remark: G: startup seed; H: packed zone and I: sludge zone at C1; J: packed zone and, K: sludge zone at C2; L: packed zone and M: sludge zone at C3.
