The Inhibitory Effect of Prunella vulgaris L. on Aldose Reductase and Protein Glycation

Table 3

Inhibitory effect of the compounds on the galactitol accumulation in rat lenses and erythrocyte.

Compounds	Rat erythrocyte galactitol content (𝜇M)^a	Galactitol content 𝜇g/lens wet weight (g)^b
Galactitol-free	0.98 ± 0.04	—
Control	22.8 ± 0.35	844.05 ± 6.89
Quercetin^c	16.15 ± 0.39	448.81 ± 3.38
Rosmarinic; acid (4)	15.05 ± 0.30	532.38 ± 6.56
Caffeic; acid ethylene; ester (5)	15.89 ± 0.41	653.76 ± 4.68

^aErythrocyte was incubated in a Krebs-Ringer bicarbonate buffer containing 30 mM galactose and in the presence or absence of 5 𝜇g/mL compounds. ^bMean of 3 duplication analyses of rat lens with compounds at a concentration of 5 𝜇g/mL. ^cQuercetin was used as positive control. Inhibitory effect was expressed as mean ± SD of triplicate experiments.