Sunflower Oil Supplementation Has Proinflammatory Effects and Does Not Reverse Insulin Resistance in Obesity Induced by High-Fat Diet in C57BL/6 Mice
Figure 2
Nitric oxide and cytokine production in peritoneal macrophages collected from mice fed with control diet (CD) or high-fat diet (HFD), supplemented or not with sunflower oil (rich in n-6 fatty acids, 2 g/Kg b.w., three times a week, by oral gavage). Animals were fed with HFD or CD for eight weeks. Peritoneal macrophages were collected and cultured for 24 h in the absence (white bars) or presence (black bars) of 2.5 μg/mL LPS. Nitric oxide (a), TNF-α (b), IL-6 (c), and IL-10 (d) were measured as described in Material and Methods. Total number of cells in peritoneal exudates was not different among the groups (data not shown). Data are presented as mean ± SEM (–10). The CD + n6 group had just 5 samples up to the detection limit in the TNF-α assay. Two-way ANOVA and Bonferroni post-test were used for statistical analysis. All LPS stimulated conditions were different from unstimulated; * versus CD with LPS; # versus CD + n6 with LPS; ° versus HFD with LPS.
