Figure 6: Effects of ginsenoside Rb1 on VEGF production (a) and HIF-1α expression (b) with or without IL-1β in HaCaT cells [26]. The human keratinocyte cell line HaCaT was treated with the indicated amounts of ginsenoside Rb1 in the presence or absence of IL-1β (20 ng/mL) for 1 or 2 h. VEGF levels in the medium were measured using a human VEGF kit. The expression of hypoxia-inducible factor (HIF-) 1α in the nuclear fraction of HaCaT cells was measured by western blot analysis with mouse anti-HIF-1α and anti-β-actin antibodies. Values are the mean ± SE for six experiments. *Significantly different from control, 𝑃 < 0 . 0 5 .