Effects of ginsenoside Rb1 on skin thickness in chronic UVB-irradiated male hairless (HRM-1) mice [31]. The initial dose of UVB was set at 36 mJ/cm2, which was subsequently increased to 54 mJ/cm2 at weeks 1–4, 72 mJ/cm2 at weeks 4–7, 108 mJ/cm2 at weeks 7–10, and finally 122 mJ/cm2 at weeks 10–12 in male albino hairless HOS: HR-1 mice. The frequency of UVB irradiation was set at three times per week. Ginsenoside Rb1 (100 fg, 10 pg, and 1 ng/mouse) was applied topically to the dorsal region of each mouse every day for 12 weeks. The dorsal skin of the hairless mice was lifted up by pinching gently under anesthetization with pentobarbital, and skin-fold thickness was measured using a Quick Mini caliper. Skin thickness after UVB irradiation was measured every week. Values are the mean ± SE for 6 mice. *Significantly different from vehicle-treated mice, .
