Review Article
Effects of Ginsenoside on Skin Changes
Table 4
Effects of ginsenoside Rb1 on the thickness of the epidermis and extracellular matrix (ECM) of the dermis at week 12 in UVB-irradiated hairless mice [31].
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The initial dose of UVB was set at 36 mJ/cm2, which was subsequently increased to 54 mJ/cm2 at weeks 1–4, 72 mJ/cm2 at weeks 4–7, 108 mJ/cm2 at weeks 7–10, and finally to 122 mJ/cm2 at weeks 10–12 in male albino hairless HOS: HR-1 mice. The frequency of UVB irradiation was set at three times per week. Ginsenoside Rb1 (100 fg, 10 pg, and 1 ng/mouse) was applied topically to the dorsal region of each mouse every day for 12 weeks. The dorsal skin samples (about 3 cm2) removed at week 12 were fixed in 10% buffered formalin, embedded in paraffin, sectioned at 5 μm thickness, deparaffinized, and stained with hematoxylin-eosin (HE) and Azan. Four different microscopic fields (×200 magnification) per plate were photographed. The thickness of the epidermis and dermis thickness were measured from the samples stained by HE and Azan, using a Digimatic Caliper. Values are the mean ± SE for 6 mice. *Significantly different from UVB-irradiated hairless mice (control), . |