Figure 1: A representative piece of esophagus before (bottom) and after (top) decellularization (a). (b) and (c) show the tissue morphology before (b) and after decellularization (c) of porcine esophagus. Acellular esophagus scaffolds showed anatomical intact structures, optimal matrix geometry, and no remaining cellular structures. Connective tissue was stained blue, chromatin red, muscle cells orange, and erythrocytes red. Magnification: ×40, bar = 100 μm. Tmus: Tunica muscularis; Ts: Tela submucosa; Tmuc: Tunica mucosa.