Cytotoxicity of Bacterial Metabolic Products, including Listeriolysin O, on Leukocyte Targets

Table 2

Survival of Jurkat cells treated with different concentrations of L. monocytogenes strain supernatants.

Strain		Supernatant concentrations
		1%	2%	3%	4%	5%	10%	20%	30%	40%	50%
1189	Median	97,5%	96,0%	97,5%	94,0%	92,5%	70,0%	64,0%	49,0%	13,0%	2,0%
	P25–P75	96,5–98,3	93,3–98,5	92,3–99,0	89,3–98,5	84,3–98,0	56,3–85,8	49,8–78,5	34,8–63,5	0,0–38,0	0,0–18,0
1190	Median	99,0%	98,0%	95,5%	95,0%	93,5%	81,0%	81,5%	64,0%	19,0%	11,0%
	P25–P75	98,8–99,3	96,0–100,3	92,0–99,3	91,3–98,3	90,0–96,0	76,8–86,8	72,0–86,8	50,0–78,0	0,0–47,5	0,0–32,0
10403S	Median	97,5%	84,0%	83,0%	71,0%	67,0%	58,5%	57,5%	30,5%	26,0%	10,5%
	P25–P75	90,3–101,0	71,5–94,8	63,8–98,8	45,0–95,8	42,3–93,0	49,0–68,8	43,5–73,5	16,3–55,5	18,0–41,8	0,0–33,0
1044	Median	100,5%	101,0%	101,0%	100,5%	100,5%	100,0%	99,5%	99,0%	98,0%	98,0%
	P25–P75	99,8–101,3	100,8–101,3	100,5–101,3	100,0–101,0	99,8–101,0	99,8–100,3	97,8–101,0	97,0–101,0	94,8–101,0	93,8–101,0

The supernatant of the 1044 mutant strain was used as a control sample not containing LLO. The percent given is that of living cells in the PI assay with different final supernatant concentrations of 1–50%, following incubation for 30 min at 22^°C (median values, P25–P75, 𝑛=4). The incubation medium contained 4% inactivated NCS.