Role of TGF-β1/Smad3 Signaling Pathway in Secretion of Type I and III Collagen by Vascular Smooth Muscle Cells of Rats Undergoing Balloon Injury

Figure 3

Expressions of type I and III collagen in VSMCs transfected with adenovirus expressing antisense Smad3 in vitro. Total RNA was extracted for RT-PCR. The culture medium was collected, and ELISA was performed to measure the contents of type I and III collagen. (a) mRNA expression of type I and III collagen in three groups; (b) contents of type I and III collagen in three groups. The mRNA expression of type I and III was normalized to that of 𝛽-actin as the relative expression. Data were expressed as mean ± SD. ^▲𝑃<0.05, ^■𝑃<0.01 versus negative control group.

(a)

(b)