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Journal of Biomedicine and Biotechnology
Volume 2012, Article ID 976972, 10 pages
http://dx.doi.org/10.1155/2012/976972
Research Article

Prevalence of Adhesion and Regulation of Biofilm-Related Genes in Different Clones of Staphylococcus aureus

1Laboratory of Medical Microbiology and Parasitology, Faculty of Medicine and Health Science, Universiti Putra Malaysia, Selangor 43400 Serdang, Malaysia
2Department of Medical Microbiology, Basrah University, Basrah, Iraq
3Laboratory of Marine Science and Aquaculture, Institute of Bioscience, Universiti Putra Malaysia, Selangor 43400 Serdang, Malaysia
4Department of Microbiology and Immunology, Arak University of Medical Sciences, Arak, Iran
5Department of Obstetrics and Gynaecology, Faculty of Medicine and Health Science, Universiti Putra Malaysia, Putra Malaysia, Selangor 43400 Serdang, Malaysia

Received 25 February 2012; Revised 10 March 2012; Accepted 4 April 2012

Academic Editor: Daniele Daffonchio

Copyright © 2012 Salman Sahab Atshan et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Clinical information about genotypically different clones of biofilm-producing Staphylococcus aureus is largely unknown. We examined whether different clones of methicillin-sensitive and methicillin-resistant S. aureus (MSSA and MRSA) differ with respect to staphylococcal microbial surface components recognizing adhesive matrix molecules (MSCRAMMs) in biofilm formation. The study used 60 different types of spa and determined the phenotypes, the prevalence of the 13 MSCRAMM, and biofilm genes for each clone. The current investigation was carried out using a modified Congo red agar (MCRA), a microtiter plate assay (MPA), polymerase chain reaction (PCR), and reverse transcriptase polymerase chain reaction (RT-PCR). Clones belonging to the same spa type were found to have similar properties in adheringto thepolystyrene microtiter plate surface. However, their ability to produce slime on MCRA medium was different. PCR experiments showed that 60 clones of MSSA and MRSA were positive for 5 genes (out of 9 MSCRAMM genes). icaADBC genes were found to be present in all the 60 clones tested indicating a high prevalence, and these genes were equally distributed among the clones associated with MSSA and those with MRSA. The prevalence of other MSCRAMM genes among MSSA and MRSA clones was found to be variable. MRSA and MSSA gene expression (MSCRAMM and icaADBC) was confirmed by RT-PCR.