BioMed Research International

Research Article

Prevalence of Adhesion and Regulation of Biofilm-Related Genes in Different Clones of Staphylococcus aureus

Table 4

Comparison of gene expression levels among 6 different clones of spa types by measuring the band intensities of RT-PCR amplicons using nanodrop spectrophotometer (ng/μL).
gene aMLST
ST-121
spa-t3204		 aMLST
ST-121
spa-t14413		 aMLST
ST-121
spa-t159		 bMLST
ST-239
spa-t4150		 bMLST
ST-239
spa-t421		 bMLST
ST-239
spa-t037
icaA 5 9 8 . 1 1 ± 1 . 0 6 6 9 8 . 1 9 ± 3 . 2 1 8 1 5 . 0 4 ± 3 . 1 7 9 6 . 1 9 ± 1 . 0 1 7 1 6 . 0 5 ± 0 . 7 4 5 1 5 . 0 6 ± 0 . 5 9
icaD 5 7 7 . 8 7 ± 1 2 . 1 4 6 7 5 . 7 6 ± 2 . 0 1 9 9 1 . 5 5 ± 1 . 1 8 7 5 . 8 8 ± 0 . 8 8 9 2 . 4 1 ± 2 . 3 3 6 9 1 . 3 7 ± 0 . 7 8
icaB 7 0 8 . 8 8 ± 2 . 0 4 8 0 3 . 7 7 ± 2 . 3 1 7 6 4 . 1 3 ± 0 . 8 6 0 1 . 8 7 ± 0 . 4 5 8 6 5 . 5 2 ± 1 . 0 2 8 6 4 . 4 8 ± 2 . 8 6
icaC 5 9 1 . 9 5 ± 1 . 8 6 9 9 . 8 8 ± 3 . 0 2 6 0 0 . 6 4 ± 1 2 . 1 9 9 8 . 9 4 ± 0 . 6 6 9 0 0 1 . 6 5 ± 1 . 5 2 5 6 7 . 5 9 ± 3 . 2 2
fnbA 6 8 4 . 5 6 ± 5 . 7 7 8 5 . 4 9 ± 1 . 3 3 9 0 4 . 9 5 ± 1 4 . 1 4 8 8 . 5 5 ± 2 . 4 1 8 0 5 . 8 6 ± 1 . 1 0 5 0 4 . 7 1 ± 1 . 8 1
fnbB c 7 4 9 . 7 2 ± 2 . 1 1 9 0 9 . 1 7 ± 4 . 2 8 0 0 . 8 4 ± 1 . 2 3
Fib 5 4 4 . 3 5 ± 2 1 . 0 1 6 4 3 . 7 2 ± 9 . 0 2 7 8 3 . 1 3 ± 1 . 9 9 4 9 . 3 7 ± 3 . 0 1 6 8 1 . 7 8 ± 3 . 1 1 4 8 3 . 4 2 ± 1 2 . 1
cna 5 6 0 . 8 5 ± 1 4 . 1 6 6 1 . 9 3 ± 1 . 6 0 6 6 0 . 3 5 ± 0 . 6 3 6 0 . 8 4 ± 0 . 8 5 5 9 . 8 9 ± 2 . 8 1 3 6 0 . 8 1 ± 9 . 2 2
eno 4 6 1 . 0 6 ± 5 . 1 5 6 5 . 1 5 ± 1 1 . 5 5 5 8 . 2 8 ± 0 . 4 4 6 4 . 0 5 ± 0 . 7 7 4 5 4 . 9 2 ± 0 . 6 2 4 5 8 . 9 4 ± 0 . 9 6
ebps 5 4 2 . 1 8 ± 1 . 3 6 4 1 . 2 8 ± 2 . 1 2 5 0 6 . 7 8 ± 0 . 8 8 5 4 3 . 1 7 ± 9 . 1 4 0 5 . 1 4 ± 0 . 7 3 8 0 6 . 3 7 ± 0 . 8 9
bbp d 5 8 0 . 5 2 ± 2 . 4 6 8 4 . 3 9 ± 2 . 1 1 4 7 4 . 1 3 ± 2 . 0 3
clfA 9 5 5 . 2 6 ± 1 1 . 8 1 0 5 1 . 4 9 ± 1 . 0 1 4 6 6 . 4 5 ± 2 . 0 1 5 5 2 . 2 9 ± 0 . 3 3 7 6 5 . 5 4 ± 2 . 3 9 7 6 6 . 6 8 ± 0 . 9 9
clfB 7 5 9 . 4 4 ± 2 . 1 8 5 9 . 6 7 ± 2 . 1 0 4 5 9 . 5 0 ± 1 . 0 4 7 5 5 . 4 1 ± 2 . 6 2 5 5 6 . 6 7 ± 0 . 5 9 6 5 9 . 8 1 ± 1 . 7 8
aThree clones of MLST sequence type ST121 belonging to clonal cluster 121 (CC121) with different spa types were showed to be weakly adherent to the surface of polystyrene microtiter plat after 48 h growth, these clones were found positive for 12 genes except the fnbB genec. bThree clones of MLST sequence type ST239 belonging to clonal cluster 8 (CC8) with different spa types were showed to be strongly adherent to the surface of polystyrene microtiter plat after 48 h growth, these clones were found positive for 12 genes except the bbp gened. Every six different clones showed statistically significant difference ( 𝑃 ≤ 0 . 0 5 ) in the intensity of RT-PCR amplified products when fewer cycles were used (25) in the linear phase of amplification for most transcripts. The replicates of Rt-PCR products were done in duplicate.