Figure 3: Muscle activity does not alter the subcellular distribution of Flag-NT-PGC-1α. (a) Representative images of Flag-NT-PGC-1α in a control fiber (control) and in another fiber stimulated continuously at 10 Hz for 4 h (stimulation). (b) The n/c fluorescence ratio of Flag-NT-PGC-1α in muscle fibers with (stimulation) or without (control) electrical stimulation. n/c values from 20 nuclei from 20 randomly selected different fibers were averaged to give the mean value for each group. (c) Representative images of NFATc1-GFP in a control fiber (control) and in another fiber stimulated continuously at 10 Hz for 4 h (stimulation). Scale bars, 10 μm for (a) and (c). (d) Representative Western blots showing the effect of muscle activity on p38 MAPK activation. Protein expression was quantified and averaged from 3 independent treatments. Phos p38, phospho-p38 MAPK; total p38, total p38 MAPK. Asterisk indicates statistical significance between groups at .