Research Article
Chemical Modifications of PhTX-I Myotoxin from Porthidium hyoprora Snake Venom: Effects on Structural, Enzymatic, and Pharmacological Properties
Table 1
Amino acid composition of native PhTX-I and their chemically modified derivatives.
| | Amino acid | PhTX-I nativea | PhTX-I modifiedb | PhTX-I modifiedc | PhTX-I modifiedd | PhTX-I modifiede |
| | Asp | 11 | 10.92 (11) | 10.9 (11) | 10.72 (11) | 10.56 (11) | | Glu | 6 | 5.98 (6) | 6.12 (6) | 5.92 (6) | 6.37 (6) | | Ser | 3 | 3.37 (3) | 3.15 (3) | 3.21 (3) | 3.03 (3) | | Gly | 11 | 11.43 (11) | 11.25 (11) | 11.39 (11) | 11.13 (11) | | His | 2 | 1.74 (2) | 1.94 (2) | 1.6 (2) | 0.65 (1) | | Arg | 7 | 7.62 (7) | 6.56 (7) | 7.15 (7) | 7.42 (7) | | Thr | 6 | 6.34 (6) | 6.09 (6) | 6.35 (6) | 6. 34 (6) | | Ala | 5 | 4.81 (5) | 5.29 (5) | 5.34 (5) | 5.44 (5) | | Pro | 6 | 5.82 (6) | 6.01 (6) | 6.05 (6) | 5.74 (6) | | Tyr | 10 | 10.24 (10) | 9.79 (10) | 5.94 (6) | 10.21 (10) | | Val | 3 | 3.33 (3) | 3.34 (3) | 3.49 (3) | 3.49 (3) | | Met | 1 | 1.47 (1) | 1.17 (1) | 1.18 (1) | 1.45 (1) | | Cys | 14 | 13.96 (14) | 13.98 (14) | 13.74 (14) | 13.69 (14) | | Ile | 2 | 2.47 (2) | 2.35 (2) | 2.27 (2) | 2.22 (2) | | Leu | 8 | 8.29 (8) | 8.44 (8) | 8.12 (8) | 8.01 (8) | | Phe | 4 | 3.58 (4) | 4.49 (4) | 4.74 (4) | 4.21 (4) | | Lys | 17 | 10.02 (10) | 16.93 (17) | 17.35 (17) | 17.2 (17) | | Trp | 3 | ND | ND | ND | ND |
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aAmino acid sequence. Amino acid analysis of chemical modifications with bAA (Lys), cNPSC (Trp), dNBSF (Tyr), and eBPB (His). ND: nondetermined.
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