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BioMed Research International
Volume 2013 (2013), Article ID 109580, 5 pages
http://dx.doi.org/10.1155/2013/109580
Research Article

A Comparative Histopathology, Serology and Molecular Study, on Experimental Ocular Toxocariasis by Toxocara cati in Mongolian Gerbils and Wistar Rats

1Department of Parasitology and Mycology, School of Medicine, Alborz University of Medical Sciences, P.O. Box 31485561, Karaj, Iran
2Department of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, P.O. Box 713451735, Shiraz, Iran
3Basic Sciences in Infectious Diseases Research Center, School of Medicine, Shiraz University of Medical Sciences, P.O. Box 713451735, Shiraz, Iran
4Department of Microbiology, School of Medicine, Birjand University of Medical Sciences, P.O. Box 917751365, Birjand, Iran
5Department of Parasitology, Faculty of Health Sciences, Kobe University Graduate School of Health Sciences, P.O. Box 6540142, Kobe, Japan
6Department of Pathology, School of Veterinary Medicine, Shiraz University, P.O. Box 713451735, Shiraz, Iran
7Department of Ophthalmology, Khalili Hospital, Shiraz University of Medical Sciences, P.O. Box 713451735, Shiraz, Iran

Received 27 April 2013; Revised 22 July 2013; Accepted 29 July 2013

Academic Editor: Christen Rune Stensvold

Copyright © 2013 Mohammad Zibaei et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The aim of this study was to compare the performance of three in-house diagnostic tests, that is, histopathology, enzyme-linked immunosorbent assay (ELISA), and polymerase chain reaction (PCR), for the diagnosis after experimental infection with Toxocara cati. Twenty Mongolian gerbils and Wistar rats were divided into ten groups ( /group). Toxocara cati infections were established in Mongolian gerbils and Wistar rats by administering doses of 240 and 2500 embryonated Toxocara cati eggs by gavage, respectively. Tissue sections were stained with Haematoxylin and Eosin and observed under the light microscope. Sera and vitreous fluid collected from separate infected groups were tested against Toxocara cati antigens, for 92 days postinfection. Genomic DNA was extracted from formalin-fixed paraffin-embedded (FFPE) blocks, and aqueous fluids belong to the animals. The histopathology test gave negative results among the groups of animals examined between 5 and 92 days postinfection. The ELISA results showed that anti-Toxocara antibodies have risen between 7 and 61 days postinfection in sera and vitreous fluid in the animals infected, respectively. Analysis of PCR products revealed positive band (660 bp) in the orbital tissue infected Mongolian gerbils at 5 days postinfection. Of the three evaluated methods, the PCR could be recommended for scientific and laboratory diagnoses of toxocariasis in experimentally infected animals.