HOXA4 Gene Promoter Hypermethylation as an Epigenetic Mechanism Mediating Resistance to Imatinib Mesylate in Chronic Myeloid Leukemia Patients

Figure 1

Derivative Melt peak of the serial percentage of methylation control produced two specific peaks which represent the unmethylated (approximately 77°C) and methylated (approximately 80°C) PCR product. Fully unmethylated sample produced only unmethylated peak, 100% methylated sample produced only methylated peak and samples with mixture of unmethylated, and methylated displayed both peaks.