HOXA4 Gene Promoter Hypermethylation as an Epigenetic Mechanism Mediating Resistance to Imatinib Mesylate in Chronic Myeloid Leukemia Patients

Figure 2

HRM curves for HOXA4 methylation standard. (A) Normalized melt curve of HOXA4 methylation standards in the form of serial methylation percentage (0% black lines, 10% pink line, 25% green line, 50% red line, 75% dark green line, and 100% orange line. (B) Melting curves were normalized to the 0% methylation standards and the standard melt curve was used as the marker for identifying the methylation percentage of samples. For example: (a) ~25% methylated, (b) ~50% methylated, and (c) 75%–100% methylated samples.