BioMed Research International / 2013 / Article / Tab 3

Research Article

Development of a Novel Reference Plasmid for Accurate Quantification of Genetically Modified Kefeng6 Rice DNA in Food and Feed Samples

Table 3

Copy number concentrations and ratios of the third sample (S3) as determined by dPCR.

Kefeng6gos9ConcentrationRatio
Positive partitionsEstimated copiesAverage NumberRSD (%)Positive partitionsEstimated copiesAverage number RSD (%)Mean (cp/ L)RSD (%)KF6/gos9

494786 794 2.6476774 790 3.3 792 2.87 1.0050.786
471780 494821
516823 492784
465797 455769
481811 446767
455766 488825

The average number was calculated from six replicate measurements with three parallel panels per chip ( ), and this value was adjusted to account for the gravimetrically prepared PCR solutions used in the Kefeng6 and gos9 assays. RSD: relative standard deviation; : probability.

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