A Pipeline with Multiplex Reverse Transcription Polymerase Chain Reaction and Microarray for Screening of Chromosomal Translocations in Leukemia
Results from the multiplex RT-PCR combined with microarray for positive controls. HL-60 and H2O were used as negative controls. (a) AML1-ETO in the cell line KASUMI-1. (b) BCR-ABL p210, b3a2 in the cell line K-562. (c) AML patient t(15;17) (PML-RARA) S-form. (d) ALL patient t(1;19) (E2A-PBX1). (I) Multiplex RT-PCR analysis. (II) Microarray analysis. (III) Histogram of data obtained from microarray. The solid line indicated the cutoff value (15% of biotinylated control probe signal intensity). The dashed line indicated the background value (negative samples and blank control plus 3x SD). Signals over both the cutoff and background control were recognized as true signals (in red circle). (IV) Sequence of multiplex RT-PCR products.
(a) KASUMI-1 AML1-ETO
(b) K-562 BCR-ABL p210, b3a2
(c) AML patient t(15;17) PML-RARA S-form
(d) ALL patient t(1;19) E2A-PBX1
Article of the Year Award: Outstanding research contributions of 2020, as selected by our Chief Editors. Read the winning articles.