Nonviral Gene Targeting at rDNA Locus of Human Mesenchymal Stem Cells

Table 1

Gene targeting in HT1080 cells.

Exp.	N	C	T	S	ATF	RTE
1	1.0	210	6	12	105	50.0%
2	3.0	529	16	30	94	53.3%

Exp.: experiment performed. N: number of cells nucleofected (×10⁶). C: total number of resistant colonies obtained from each experiment. S: number of colonies screened. T: number of colonies screened as targeted recombinants. ATF: absolute targeting frequency (×10⁻⁶) = TC/NS. RTE: relative targeting efficiency = T/S.