Nonviral Gene Targeting at rDNA Locus of Human Mesenchymal Stem Cells

Table 2

Gene targeting in MSCs.

Don	N	C	S	T	ATF	RTE
1#	0.5	17	9	2	7.6	22.2%
2#	1.0	36	23	3	4.7	13.0%
2#	3.0	98	50	11	7.2	22.0%

Don: donor of bone marrow. N: number of cells nucleofected (×10⁶). C: total number of resistant colonies obtained from each experiment. S: number of colonies screened. T: number of colonies screened as targeted recombinants. ATF: absolute targeting frequency (×10⁻⁶) = TC/NS. RTE: relative targeting efficiency = T/S. 1#, 2#: bone marrow donors.