Engineered Hypopharynx from Coculture of Epithelial Cells and Fibroblasts Using Poly(ester urethane) as Substratum
Figure 4
The morphology of epithelial cells which were cultured on the scaffold of control PEU (a) and PEU-SF (b) for 7 d (1) and 14 d (2), respectively. The scaffold was cut to fit in 96-well culture plate. Cells were cultured at 37°C in humidified air with 5% CO2 (the same conditions were followed for all cell culture). The seeding density is 5 × 105/mL and 50 μL cell suspension was used for each well. Samples were observed under SEM.
