Gamma Knife Surgery as Monotherapy with Clinically Relevant Doses Prolongs Survival in a Human GBM Xenograft Model
Figure 4
H/E and IHC staining of GBM xenografts. H/E staining of the tumors (a) which appear hypercellular, but with more prominent angiogenesis and necrosis in the pB GBM xenografts (lower panels). Granulomatous cerebral angiitis with polynuclear macrophages in the perivascular space can be seen in the pA GBM xenograft treated with 18 Gy (Upper right panel). Scale bars: 50 μm. IHC and TUNEL staining (brown) show robust expression of Nestin, Vimentin, and Ki-67 and TUNEL positive cells. Panels at low magnification show Nestin positive cells in the pA xenograft migrating along the Corpus Callosum (b). Scale bars pA: Nestin 250 μm, Vimentin: 100 μm, Ki-67 from pA ctrl: 100 μm, all other panels: 25 μm. Scale bars pB: Nestin and Vimentin: 100 μm, all other panels: 50 μm.
