Generation of Leukemia Inhibitory Factor-Dependent Induced Pluripotent Stem Cells from the Massachusetts General Hospital Miniature Pig

Figure 2

Integration of transgene and pluripotent gene expression in porcine iPSCs. Genomic PCR was conducted to confirm the integration of transcription factors, while exogenous or endogenous gene expression was identified using RT-PCR. Both two lines had integrated all six transcription factors, which were expressed exogenously in further culture. Furthermore, all six pluripotent genes were endogenously expressed in both A10 and A15 at passage 20.