Generation of Leukemia Inhibitory Factor-Dependent Induced Pluripotent Stem Cells from the Massachusetts General Hospital Miniature Pig
In vitro and in vivo differentiation of porcine iPSCs. (a) Embryoid bodies were cultured in stem cell media without LIF and bFGF for 10 days. (b) RT-PCR analysis shows that all differentiation makers for the three germ layers were expressed in the EBs of A15 and A10, but mesoderm markers, T (brachyury) and Criptic, were not expressed in those of A10. To test the in vivo differentiation of porcine iPSCs, A10 and A15 lines were injected subcutaneously into a nude mouse. Only the A15 cell line formed teratomas, which were dissected 9 weeks after injection. Hematoxylin-eosin staining shows three types of tissues: (c) mesoderm, cartilage tissue; (d) ectoderm, neuronal tissue; (e) endoderm, glandular epithelium.