BioMed Research International / 2013 / Article / Tab 1 / Research Article
Generation of Leukemia Inhibitory Factor-Dependent Induced Pluripotent Stem Cells from the Massachusetts General Hospital Miniature Pig Table 1 Primer sets for RT- and real-time qPCR.
Gene name Primer Sets (5′→3′) References Forward Reverse For RT-PCR* hPOU5F1 GATCAAGCAGCGACTATGCA TCTGGGATGGAAACTGGAA Ezashi et al. 2009 [5 ] hKLF4 CTGCGGCAAAACCTACACAA Ezashi et al. 2009 [5 ] hNANOG CCACTAGGTATTTTAGTACTCC NM_024865 hSOX2 CCTGGCATGGCTCTTGGC Ezashi et al. 2009 [5 ] hC-MYC GATTCTCTGCTCTCCTCGACG Ezashi et al. 2009 [5 ] hLIN28 GCGGCCAAAAGGAAAGAGCA NM_024674 Gapdh CTCAACGACCACTTCGTCAA TCTGGGATGGAAACTGGAAG X94251 pPou5f1 ACAAGGAGAAGCTGGAGCCG CGCGGACCACATCCTTCTCT NM001113060 pKlf4 TGGGCAAGTTTGTGTTGAAG AGGAAGGGTGGGTAGTTTGG DQ000310.1 pNanog TGAGGTTTATGGGCCTGAAG ATTTCATTCGCTGGTTCTGG NM_001129971.1 pSox2 CAAGATGCACAACTCGGAGA TGCTGTAGCTGCAGTTGCTC NM_001123197.1 pc-Myc CAGATCAGCAACAACCGAAA TCCAACTCTGGGATCTGGTC FJ882404.1 pLin28 TGCACCAGAGTAAGCTGCAC CTGCATATTCTTCCCCTTGG HM347046.1 For RT-qPCR Pou5f1 AGCGCTTCAGAAAGATCTCG GAGCTGCAAAGCCTCAAAAC NM001113060 Nanog GATTGGGGTGGTTAGCTCCT TGAAGGTGAGACTCGCTCTG NM_001129971.1 Sox2 CAGGAGGGAAGACTCCATCA CTCCCTCTTGGACAGTCGAG NM_001123197.1 Klf4 GCCCTTAGAGGCCCACTT GCAGGGCAGGATGACAGT DQ000310.1 Rex1 AGCTAACCCTGTCCACATCG CAAGTCAGCAGCAGTCTCCA XM_003123016.2 Socs3 CTCCGACTGAACCCTCCTC CGTTGACTGTTTTCCGACAG HM045422.1
The reverse primer used for exogenous human POU5F1 , KLF4 , NANOG , SOX2 , C-MYC , and LIN28 expression is a part of woodchuck hepatitis virus posttranscriptional regulatory element (WPRE) region within viPS lentiviral vector. h: human; p: pig; F: forward; R: reverse.