Figure 3: (a) Gene expression profiles of pCAGGS-BMP4 transfected cells cultured in micromass at day 3. (b) Western blot analysis of culture medium for BMP4 at day 3. (c) Immunostaining of micromass for BMP4 at day 3. BMP4 gene expression levels were not significantly altered by LiCl. However, BMP4 protein accumulation in both culture medium and cells was dose dependently decreased by LiCl. Aggrecan and Col2a1 mRNA expression was decreased by LiCl. BMP antagonist, noggin, gremlin 1 and follistatin showed different responses to LiCl. The mRNA expression of cathepsin H was up-regulated by LiCl. , .