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BioMed Research International
Volume 2013, Article ID 146716, 10 pages
Research Article

Inhibition of Carrageenan-Induced Acute Inflammation in Mice by Oral Administration of Anthocyanin Mixture from Wild Mulberry and Cyanidin-3-Glucoside

1Laboratório de Química e Bioquímica e Biologia Molecular de Alimentos, Departamento de Alimentos e Nutrição Experimental, FCF, Universidade de São Paulo, Avenida Professor Lineu Prestes 580, Bloco 14, 05508-000 São Paulo, SP, Brazil
2Núcleo de Apoio à Pesquisa em Alimentos e Nutrição (NAPAN), Universidade de São Paulo, 05508-000 São Paulo, SP, Brazil
3Laboratório de Farmacologia, Unidade de Inflamação, Instituto Butantan, Avenida Vital Brasil 1500, 05503-000 São Paulo, SP, Brazil

Received 6 September 2012; Accepted 9 December 2012

Academic Editor: José Carlos Tavares Carvalho

Copyright © 2013 Neuza Mariko Aymoto Hassimotto et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Anthocyanins are flavonoids which demonstrated biological activities in in vivo and in vitro models. Here in the anti-inflammatory properties of an anthocyanin-enriched fraction (AF) extracted from wild mulberry and the cyanidin-3-glucoside (C3G), the most abundant anthocyanin in diet, were studied in two acute inflammation experimental models, in the peritonitis and in the paw oedema assays, both of which were induced by carrageenan (cg) in mice. In each trial, AF and C3G (4 mg/100 g/animal) were orally administered in two distinct protocols: 30 min before and 1 h after cg stimulus. The administration of both AF and C3G suppresses the paw oedema in both administration times ( ). In the peritonitis, AF and C3G reduced the polymorphonuclear leukocytes (PMN) influx in the peritoneal exudates when administered 1 h after cg injection. AF was more efficient reducing the PMN when administered 30 min before cg. Both AF and C3G were found to suppress mRNA as well as protein levels of COX-2 upregulated by cg in both protocols, but the inhibitory effect on PGE2 production in the peritoneal exudates was observed when administered 30 min before cg ( ). Our findings suggest that AF and C3G minimize acute inflammation and they present positive contributions as dietary supplements.