A Recombinant Multiepitope Protein for Hepatitis B Diagnosis
Figure 5
Heat-induced unfolding of rMEHB by circular dichroism (CD). (a) Far UV CD spectra in 2 mM MOPS buffer pH 7.0 and (b) pH 8.0, as a function of temperature. Molar ellipticities were measured from 260 to 195 nm at temperature rising from 25°C to 95°C. The arrows indicate decreased dichroic signal at 200 nm with increased temperature. (c) Temperature induced unfolding curves of rMEHB in 2 mM MOPS buffer pH 7.0 (--) and pH 8.0 (—) monitored at 200 nm.
