Figure 3: Atomic force microscopy analysis of the effects of glutamine and alanyl-glutamine supplementation on IEC-6 cell morphology following damage induced by TcdA. Effect of alanyl-glutamine (TcdA + Ala-Gln) or glutamine supplementation (TcdA + Gln), both at 10 mM, simultaneously with 24-hour exposure to TcdA at 10 ng/mL on IEC-6 cell. The images were confectioned with AFM in the contact mode with scanning of μm2. Control (a), TcdA (b), TcdA + Ala-Gln (c), and TcdA + Gln (d). The height (e), the area (f), and volume (g) were calculated with the nanoscope 5.30R3.SR3 software. compared to control. compared to TcdA.