Strategies to Characterize Fungal Lipases for Applications in Medicine and Dairy Industry
Figure 3
Agar plate screening for lipases. Using the substrates (a) Tween, (b) tributyrin, and (c) vegetable oil. In the Tween method, formation of calcium crystals was observed. The tributyrin method shows a clear zone, whereas in the Rhodamine method, formation of fluorescence with fatty substrate was observed under UV illumination. Active zones are increasing with a period of incubation time and these zones can be measured.
