1Click1View: Interactive Visualization Methodology for RNAi Cell-Based Microscopic Screening

Table 2

Key features of the datasets used in the experiments.

Screen	Description
(1) Biogenesis Screen (BGS)	This screen studied the biogenesis of the small ribosomal subunit in human cells (40S subunit). Towards this, an assay for visual detection of nuclear maturation defects of the 40S subunit was developed. In the cellular process, a HeLa cell line bearing a fluorescently tagged ribosomal protein of the 40S subunit (Rps2-YFP), which is expressed only upon induction, was used to selectively visualize newly synthesized 40S subunits. This assay has been developed to detect nuclear 40S maturation defects upon depletion of a protein by RNAi. This allowed to identify proteins functioning in 40S biogenesis in human cells and, according to the classification, assign their requirement to nucleolar or nucleoplasmic maturation events.; Screening results data for QC: 76747 data points corresponding to individual siRNA oligonucleotide (4 siRNAs per targeted gene), including 4 numeric parameters and gene/siRNA annotation.
(2) miRNA biogenesis-Antagomirs Screen	The goal of the screen was to monitor the levels of two individual miRNAs (mir16 with GFP and mir22 with mCherry) within the cells and to investigate in modulators of the miRNA expression/function. In addition, the experiment was designed to study an inhibitor of miRNAs (Antagomir)—interesting point was to know how it is taken up and how it does act (which genes are important).; Screening results data for QC: 26880 data points corresponding to pooled siRNAs (4 oligonucleotides per targeted gene), including 5 numeric parameters and gene/siRNA annotation.