Release of IL-1β Triggered by Milan Summer PM₁₀: Molecular Pathways Involved in the Cytokine Release

Figure 7

Early endosome antigen 1 EEA1 protein expression in THP-1-derived macrophages exposed to summer Milan PM₁₀. The cells were treated with summer PM₁₀ (2.5 μg/cm²) for 30 min, 1 h, 2 h, 4 h, and 6 h and then assessed for EEA1 expression by Western analysis. Mean and s.d. of at least three independent experiments. ^∗∗∗𝑃<0.001 versus CTRL, ^∗∗𝑃<0.01 versus control, ^∗𝑃<0.05 versus CTRL, and ^#𝑃<0.05 versus 30 min.