Gemifloxacin, a Fluoroquinolone Antimicrobial Drug, Inhibits Migration and Invasion of Human Colon Cancer Cells
Figure 5
GMF inhibited inducible NF-κB activity in SW620 cells. GMF reduced TNF-α-mediated NF-κB nuclear translocation (a), activation (b), IKK phosphorylation (c), and TAK1/TAB2 interaction (d). Cells were pretreated with GMF (20 μg/mL) for 1 hour; then TNF-α (20 ng/mL) was added for another 3 hours. Protein expression was assessed by immunoblot, and protein-protein interaction was examined by immunoprecipitation. The DNA binding activity of NF-κB in the nuclear fraction was assessed by a Trans-AM ELISA kit. All results are representative of at least three independent experiments. Each value is the mean ± SD of three determinations. The asterisk (*) indicates a significant difference between the two test groups, as analyzed by Student’s t test ().
