BioMed Research International / 2013 / Article / Fig 1

Research Article

Chronic Heat Stress Inhibits Immune Responses to H5N1 Vaccination through Regulating CD4+CD25+Foxp3+ Tregs

Figure 1

Experimental design schematic. Mice were randomly divided into four groups. Mice under chronic heat stress (CHS) were exposed to °C for 4 h per day from day 0 to day 21, meanwhile mice of thermally neutral (TN) groups were maintained at °C. Mice were immunized with 50 μL formalin-inactivated H5N1 virus or PBS on day 0 and boosted on day 14, and all the mice were challenged with PBS-diluted H5N1 virus (50 PFU) on day 21. The mortality of virus-infected mice was observed for 14 days after the viral challenge. Three mice from each group were sacrificed on days 7, 14, 21, 24, and 27; serum, lung, and spleen tissues were collected.

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